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KMID : 0390020100200040226
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Pediatric Allergy and Respiratory Disease
2010 Volume.20 No. 4 p.226 ~ p.231
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Comparison of Methods for Measuring House Dust Mite Allergens
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Ha Jong-Mok
Kim Sang-Won
Lim In-Seok
Shin Mee-Yong
Han Young-Shin
Ahn Kang-Mo
Lee Sang-Il
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Abstract
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Purpose:This study was conducted to evaluate the accuracy of semiquantitative tests targeting house dust mite (HDM) allergens.
Methods:Thirty-nine dust samples were collected from the homes of 14 patients. These samples were evaluated using the following semi-quantitative tests: Rapid test for Mite Allergenⓡ, Acarexⓡ, Mitey checkerⓡ, and SD CHECK Quick Mite Testⓡ. The samples were then assayed by enzyme-linked immunosorbent assay (ELISA) for Der p 1, Der f 1 and group 2 allergens. Finally, the results from the 4 kits were compared and the correlation with ELISA was determined.
Results:Concentrations of HDM allergens determined using Acarexⓡ were associated with those of SD CHECK Quick Mite Testⓡ and Mitey checkerⓡ, (P=0.004 and 0.035, respectively; ρ=0.454 and 0.339, respectively). The concentrations determined by SD CHECK Quick Mite Testⓡ were associated with those of Mitey checkerⓡ and Rapid test for Mite Allergenⓡ (P=0.000 and 0.001, respectively; ρ=0.664 and 0.518, respectively). There was also a significant correlation between the results obtained using Mitey checkerⓡ and Rapid test for Mite Allergenⓡ (P=0.000, ρ=0.602). The Der f 1 levels were associated with the concentrations determined by SD CHECK Quick Mite Testⓡ, Mitey checkerⓡ and Rapid test for Mite Allergenⓡ (P=0.003, 0.000 and 0.005, respectively; ρ=0.467, 0.544 and 0.438, respectively). Group 2 allergen levels were associated with the concentrations determined by SD CHECK Quick Mite Testⓡ and Rapid test for Mite Allergenⓡ (P=0.019 and 0.038, respectively; ρ=0.374 and 0.334, respectively).
Conslusion:Four popular semiquantitative test kits may be adequate for detecting HDM allergens at home. However, quality assurance is needed for clinical usage because they showed variable sensitivity and specificity.
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KEYWORD
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Dermatophagoides Allergens, House Dust
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